Elements of Structural Biology

Reference books

Kensal, E. Van Holde et al., Principles of Physical Biochemistry (Second Edition)

Pearson Education International.

Cantor, C.R., and Schimmel  P.R., Biophysical Chemistry, Vols. I-III, W.H. Freeman and Co., San Francisco, USA, 1980

Research papers and reviews.

 

Suggested pre-requisite:

Physics/ Chemistry/ Mathematics at Bachelor`s degree level

 

Course Schedule (January - April)

Lectures

Topics

1-10

General introduction, review of quantum chemistry

 

Introduction to spectroscopic techniques

 

Absorption spectroscopy

 

Circular Dichroism

 

Fluorescence, anisotropy

11-20

Chromatographic methods

 

Ultra centrifugation methods

 

Hydrodynamic techniques, dynamic light scattering

 

Methods to measure protein-ligand interactions, ITC, DSC

21-30

Mass spectrometry

 

Crystallography basics

 

Space group assignments and ambiguities

 

Strategies in MR

 

MIR methods

 

MAD / SAD techniques

 

Electron density interpretation

 

Density modification techniques

31-35

X-Ray fiber diffraction

 

Solution X Ray scattering

 

Introductory Electron microscopy

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Suggested topics for presentations:

1. Peptide sequencing using mass spectrometry

2. Experimental methods for protein-ligand interactions

3. Protein Dynamics as probed by NMR methods

4. Characterization of ion channels

5. Features of membrane proteins: structural aspects and protein chemistry

6. Fitting high resolution models in low resolution EM maps

MB 303 (JAN) 3:0